Published September 3, 2010
| Supplemental Material + Accepted Version
Journal Article
Open
Preparation of Translationally Competent tRNA by Direct Chemical Acylation
- Creators
- Duffy, Noah H.
-
Dougherty, Dennis A.
Abstract
Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) using amino acid phosphate esters. The successful implementation of this methodology to full-length suppressor tRNA is described, and it is shown that the derived aminoacyl-tRNA is translationally competent in Xenopus oocytes.
Additional Information
© 2010 American Chemical Society. Received June 17, 2010. Publication Date (Web): August 5, 2010. We thank Dr. Scott Virgil (Caltech) for assistance with LC/MS, Dr. Russell Jacobs (Caltech) for suggesting DTPA, Dr. Ronald Kluger (University of Toronto) and students for helpful suggestions. This work was supported by the NIH (NS 34407).Attached Files
Accepted Version - nihms227191.pdf
Supplemental Material - ol101408f_si_002.pdf
Files
ol101408f_si_002.pdf
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Additional details
- PMCID
- PMC2929286
- Eprint ID
- 19887
- Resolver ID
- CaltechAUTHORS:20100913-092613751
- NIH
- NS34407
- Created
-
2010-09-16Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field