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Published June 1966 | Published
Journal Article Open

Demonstration of Extracellular Space by Freeze-Drying in the Cerebellar Molecular Layer


In electron micrographs of the molecular layer of the mouse cerebellum frozen within 30 sec of circulatory arrest and subsequently dried at -79 °C an appreciable extracellular space was found between the axons of the granular cells. Tight junctions were regularly observed between pre- and postsynaptic structures and the enveloping glia cells. In micrographs of cerebellum frozen 8 min after decapitation the space between the axons was absent and tight junctions between the nerve fibres were almost exclusively encountered. The extracellular space of asphyxiated and non-asphyxiated tissue in electron micrographs of frozen-dried material is similar to the space in comparable tissues treated by freeze-substitution. These observations suggest that there is an appreciable amount of extracellular material in oxygenated, living tissue which is taken up by cellular elements during asphyxiation.

Additional Information

Copyright © 1966 by Company of Biologists. Submitted on November 2, 1965. This investigation was supported, in part, by a grant from the National Science Foundation (G-21531).

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