Structural evolution of nitrogenase states under alkaline turnover

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© The Author(s) 2024.

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Acknowledgement

This work was funded by support from the Howard Hughes Medical Institute (D.C.R.), NIH 1F32GM143836 (R.A.W.), and NIH 1K99GM152765 (R.A.W.). The generous support of the Beckman Institute for the Caltech CryoEM Resource Center was essential for the performance of this research. We thank Dr. Jens Kaiser, Dr. Songye Chen, Dr. Nathan Dalleska, and Dr. Ailiena Maggiolo for their invaluable discussions.

Funding

This work was funded by support from the Howard Hughes Medical Institute (D.C.R.), NIH 1F32GM143836 (R.A.W.), and NIH 1K99GM152765 (R.A.W.).

Contributions

Conceptualization: R.A.W., D.C.R. Methodology: R.A.W. Investigation: R.A.W. Visualization: R.A.W. Supervision: R.A.W., D.C.R. Writing—original draft: R.A.W. Writing—review & editing: R.A.W., D.C.R.

Data Availability

The single particle cryoEM maps and models have been deposited into the PDB and EMDB. Datasets been deposited with the following PDB and EMDB codes: 9CJE and EMD-45629, (MoFe^{Alkaline-20sec}), 9CJD and EMD-45628, (MoFe^{Alkaline-5min}), 9CJC and EMD-45627, [https://www.ebi.ac.uk/pdbe/entry/emdb/EMD-45627] (MoFe^{Alkaline-20min}), 9CJB and EMD-45626 (MoFe^{Alkaline-60min}), and 9CJF and EMD-45630 (MoFe^{Alkaline-inactivated}-NafT complex). CryoEM maps for 3DVA subclass maps MoFe^{Alkaline-5min-B} (‘cofactor out’) and MoFe^{Alkaline-5min-D} (‘cofactor in’) are included as supplementary maps with the MoFe^{Alkaline-5min} deposition. PDB codes of previously published structures used in this study are 8ENL, 8ENO, 8CRS, and 3U7Q. Source Data are provided as a Source Data file. Source data are provided with this paper.

Supplemental Material

Supplementary Information (PDF)

Description of Additional Supplementary Files (PDF)

Supplementary Movie 1 (MP4)

Supplementary Movie 2 (MP4)

Reporting Summary (PDF)

Transparent Peer Review File (PDF)

Source Data (XLSX)