In Vivo Synthesis and Structural Analysis of Alanylglycine-Rich Artificial Proteins
A series of 22 alanylglycine-rich artificial proteins has been prepared by bacterial expression of the corresponding synthetic genes. These polymers have been isolated and purified, and subjected to structural analysis by gel electrophoresis, mass spectrometry, x-ray scattering, and vibrational and NMR spectroscopy. Strategies have been developed for the in vivo synthesis of artificial proteins containing non-natural amino acids.
© 1994 American Chemical Society. Received May 4, 1993.