Generation of patterned kidney organoids that recapitulate the adult kidney collecting duct system from expandable ureteric bud progenitors

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Acknowledgement

We would like to thank Jeffrey Boyd and Bernadette Masinsin of the USC Flow Cytometry Facility for FACS; Seth Ruffins of the USC Optical Imaging Facility for help with microscopy; Dejerianne Ostrow and David Ruble of the Children’s Hospital Los Angeles Molecular Pathology Genomics Core for RNA-seq; Meng Li, Yibu Chen, and Eddie Loh of the USC Norris Medical Library Bioinformatics Service for help with the RNA-seq computational analysis; Haruhiko Akiyama and Juan Carlos Izpisua Belmonte for sharing the Sox9-GFP mice, Naoki Nakayama for sharing the SOX9-GFP hiPSC line; Dr Melissa L. Wilson (Department of Preventive Medicine, University of Southern California) and Family Planning Associates for coordinating fetal tissue collection; and Cristy Lytal for helping with editing the manuscript. 

Funding

This work is supported by departmental startup funding and USC/UKRO Kidney Research Center funding to Z.L. J.C. was supported by CIRM Bridges Program. Z.Z. was supported by USC Stem Cell Challenge Award. Work in A.P.M.’s laboratory was supported by a Grant from the NIDDK (DK054364). E.A.R. was supported by an F31 fellowship (DK107216).

Contributions

Z.Z., B.H., A.P.M., and Z.L. designed the study. Z.Z., B.H., R.K.P., Y.L., J.C., T.P., E.A.R., A.D.K. and J.A.M. performed experiments. A.C.V. and M.E.T. performed RNA-seq computational analysis. M.E.T and B.H.G. provided human fetal kidney samples. N.M.P.-S., K.R.H., and A.P.M. provided reagents and helpful discussions. Z.Z., B.H., and Z.L. wrote the manuscript. Z.Z., B.H., J.Y., N.M.P.-S., K.R.H., A.P.M., and Z.L. edited the manuscript.

Data Availability

RNA-seq data have been submitted to Gene Expression Omnibus (GEO) with accession number “GSE149109 [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE149109].” For GUDMAP/RBK resources, visit https://www.gudmap.org. Key resources such as antibodies, chemicals, recombinant proteins, genotyping, and qRT-PCR primers are provided in Supplementary Tables 3–5. All other relevant data supporting the key findings of this study are available within the article and its Supplementary Information files or from the corresponding author upon reasonable request. A reporting summary for this Article is available as a Supplementary Information file. Source data are provided with this paper.

Conflict of Interest

Patent application has been filed on April 27, 2020 by University of Southern California on behalf of inventors Z.Z., B.H., A.P.M., and Z.L. for the mouse and human UB organoid generation, expansion, and CD organoid differentiation systems described in this study. The other authors declare no competing interests.

Supplemental Material

• Supplementary Data 1: Description: RNA-seq TMM values.
•  Supplementary Data: Description: 4-fold change gene list from RNA-seq (tip vs trunk)