Self-powered microfluidic chips for multiplexed protein assays from whole blood
Abstract
We report herein on a self-powered, self-contained microfluidic-based chip designed to separate plasma from whole blood, and then execute an assay of a multiplexed panel of plasma biomarker proteins. The power source is based upon a chemical reaction that is catalytically triggered by the push of a button on the chip. We demonstrate assays of a dozen blood-based protein biomarkers using this automated, self-contained device. This platform can potentially permit high throughput, accurate, multiplexed blood diagnostic measurements in remote locations and by minimally trained individuals.
Additional Information
© Royal Society of Chemistry 2009. Received 27th November 2008, Accepted 25th March 2009. First published on the web 16th April 2009. We would like to thank Dr. Rong Fan, Habib Ahmad, and Ke Xu for helpful discussions, and for providing assistance with certain aspects of device fabrication. This work was funded by the National Cancer Institute grant no. 5U54 CA119347 (J.R.H., P.I.), by the Institute for Collaborative Biotechnologies through grant DAAD19-03-D-0004 from the US Army Research Office, and also by the Jean Perkins Foundation.Attached Files
Published - b821247c.pdf
Accepted Version - nihms348627.pdf
Supplemental Material - QinVideo_1-peroxide_chip_blood_separation.wmv
Supplemental Material - QinVideo_2-flow_speed_variance.wmv
Supplemental Material - Qinb821247c.pdf
Files
| Name | Size | Download all |
|---|---|---|
|
md5:96ac65b70de5a1936311bbc8e14da39e
|
11.8 MB | Download |
|
md5:c4d5d5f1375b06f1d63474fde6108238
|
3.2 MB | Preview Download |
|
md5:deeee91d93ecebfe1bb73b8a6859f516
|
1.2 MB | Preview Download |
|
md5:bfc143e26e6cf637c58c7b0bf1b6a044
|
3.1 MB | Preview Download |
|
md5:595d0f8d8b3c84d81e17148c2a32a8eb
|
1.3 MB | Download |
Additional details
- PMCID
- PMC3651861
- Eprint ID
- 15716
- Resolver ID
- CaltechAUTHORS:20090910-091059453
- National Cancer Institute
- 5U54 CA119347
- Army Research Office (ARO)
- DAAD19-03-D-0004
- Jean Perkins Foundation
- Created
-
2009-10-07Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field