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Published May 2018 | Accepted Version
Journal Article Open

Light-Sheet Imaging to Elucidate Cardiovascular Injury and Repair


Purpose of Review: Real-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from zebrafish to rodent hearts. Recent Findings: Light-sheet imaging enables rapid data acquisition with high spatiotemporal resolution and with minimal photo-bleaching or photo-toxicity. We demonstrated the capacity of this novel light-sheet approach for scanning a region of interest with specific fluorescence contrast, thereby providing axial and temporal resolution at the cellular level without stitching image columns or pivoting illumination beams during one-time imaging. This cutting-edge imaging technique allows for elucidating the differentiation of stem cells in cardiac regeneration, providing an entry point to discover novel micro-circulation phenomenon with clinical significance for injury and repair. Summary: These findings demonstrate the multi-scale applications of this novel light-sheet imaging strategy to advance research in cardiovascular development and regeneration.

Additional Information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature. First Online: 24 March 2018. The authors would like to express gratitude to all lab members for discussion and insights. Compliance with Ethical Standards. Conflict of Interest: Yichen Ding, Juhyun Lee, Jeffrey J. Hsu, Chih-Chiang Chang, Kyung In Baek, Sara Ranjbarvazirj, Reza Ardehali, René R. Sevag Packard, and Tzung K. Hsiai declare no conflict of interest. Human and Animal Rights and Informed Consent: This article does not contain any studies with human subjects performed by any of the authors. All animal rights have been approved by AAALAC and USDA.

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