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Published June 22, 2023 | Published
Journal Article Open

Response to comment on 'A conserved strategy for inducing appendage regeneration in moon jellyfish, Drosophila, and mice'


Previously we reported evidence that a regenerative response in the appendages of moon jellyfish, fruit flies, and mice can be promoted by nutrient modulation (Abrams et al., 2021). Sustar and Tuthill subsequently reported that they had not been able to reproduce the induced regenerative response in flies (Sustar and Tuthill, 2023). Here we discuss that differences in the amputation method, treatment concentrations, age of the animals, and stress management explain why they did not observe a regenerative response in flies. Typically, 30–50% of treated flies showed response in our assay.

Copyright and License

© 2023, Li et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.


We thank Bruce Hay, Arjun Raj, Yujing Yang, Jesus del Rio Salgado, Judah Bates, and Aki Ohdera for feedback and discussions.


The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.


Yutian Li, Conceptualization, Data curation, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing – original draft, Writing – review and editing; Anish A Sarma, Formal analysis, Validation, Methodology, Writing – review and editing; Iris T Lee, Investigation, Writing – review and editing; Fayth Hui Tan, Michael J Abrams, Zevin J Condiotte, Martin Heithe, Misha Raffiee, John O Dabiri, David A Gold, Writing – review and editing; Lea Goentoro, Conceptualization, Resources, Data curation, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing – original draft, Project administration, Writing – review and editing

Data Availability

The raw images of the single fly tracking data are available at CaltechDATA: https://doi.org/10.22002/ D1.2157. The raw measurements of the single fly tracking data are available in: https://cdn.elifesciences.org/articles/65092/elife-65092-fig5-data1-v3.xlsx. Matlab code to process the data in the spreadsheet can be downloaded from: https://cdn.elifesciences.org/articles/65092/elife-65092-fig5- code1-v3.zip.

Conflict of Interest

Fayth Hui Tan, Michael J Abrams, Martin Heithe, Lea Goentoro: Has applied for a patent related to the findings reported in this manuscript (US Patent Application No. 17/355,727). The other authors declare that no competing interests exist.


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Additional details

November 10, 2023
January 9, 2024