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Published February 24, 2023 | Supplemental Material + Published
Journal Article Open

Neural connectivity molecules best identify the heterogeneous clock and dopaminergic cell types in the Drosophila adult brain


Our recent single-cell sequencing of most adult Drosophila circadian neurons indicated notable and unexpected heterogeneity. To address whether other populations are similar, we sequenced a large subset of adult brain dopaminergic neurons. Their gene expression heterogeneity is similar to that of clock neurons, i.e., both populations have two to three cells per neuron group. There was also unexpected cell-specific expression of neuron communication molecule messenger RNAs: G protein–coupled receptor or cell surface molecule (CSM) transcripts alone can define adult brain dopaminergic and circadian neuron cell type. Moreover, the adult expression of the CSM DIP-beta in a small group of clock neurons is important for sleep. We suggest that the common features of circadian and dopaminergic neurons are general, essential for neuronal identity and connectivity of the adult brain, and that these features underlie the complex behavioral repertoire of Drosophila.

Additional Information

© 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). We thank the members of Rosbash laboratory for critical reading and discussion of the manuscript. We appreciate L. Luo, L. Zipursky, P. Garrity, and Y. Kurmangaliyev for insightful comments on the manuscript. We also thank the Bloomington Drosophila Stock Center for stocks. This work was supported by the Howard Hughes Medical Institute. Author contributions: D.M., K.Z., and M.R. designed the study. D.M., N.H., K.C.A., and J.Q.L. performed the experiments and data analysis. D.M. and M.R. wrote the paper. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. The raw sequencing data and processed gene expression matrix have been deposited in GEO under accession number GSE198948, and the single-cell RNA sequencing data for clock neurons by plate-based method have been deposited in GEO under the accession code GSE157504. Custom codes for the analysis in the current study are available on online repositories (Zenodo: https://zenodo.org/record/7387695; GitHub: https://github.com/rosbashlab/scRNA-seq-clock-and-dopaminergic-neurons). The authors declare that they have no competing interests.

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Published - sciadv.ade8500.pdf

Supplemental Material - sciadv.ade8500_sm.pdf

Supplemental Material - sciadv.ade8500_table_s2.zip


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August 22, 2023
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