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Published August 18, 1989 | public
Journal Article

Inhibition of DNA binding proteins by oligonucleotide-directed triple helix formation


Oligonucleotides that bind to duplex DNA in a sequence-specific manner by triple helix formation offer an approach to the experimental manipulation of sequence-specific protein binding. Micromolar concentrations of pyrimidine oligodeoxyribonucleotides are shown to block recognition of double helical DNA by prokaryotic modifying enzymes and a eukaryotic transcription factor at a homopurine target site. Inhibition is sequence-specific. Oligonucleotides containing 5-methylcytosine provide substantially more efficient inhibition than oligonucleotides containing cytosine. The results have implications for gene-specific repression by oligonucleotides or their analogs.

Additional Information

© 1989 American Association for the Advancement of Science. 21 March 1989; Accepted 5 July 1989. We thank R. Tjian for pSpl-516C, and we also thank members of the Dervan and Wold groups for helpful discussions. Supported by grants from the Caltech Beckman Institute and the NIH (B.W. and P.B.D.), by a Gosney Fellowship, and an American Cancer Society Fellowship to L.J.M.

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August 19, 2023
October 19, 2023