CD9 Plays a Role in Schwann Cell Migration in Vitro

Creators: Anton, Eva S.; Hadjiargyrou, Michael; Patterson, Paul H.; Matthew, William D.

Abstract

To identify molecules that regulate Schwann cell migration, we have generated a panel of monoclonal antibodies against Schwann cell surface antigens that modulate Schwann cell migration in in vitro bioassays. One of these antibodies, SMRA1, recognizes a 26 kDa Schwann cell surface membrane protein identified here as CD9. SMRA1 enhances Schwann cell migration on two biologically relevant substrates: living axons of cultured dorsal root ganglion neurons, and cryostat sections of sciatic nerve. This CD9-induced regulation of Schwann cell motility is correlated with a rise in cytosolic calcium and enhanced tyrosine phosphorylation of several Schwann cell proteins. These results, together with the findings of Hadjiargyrou and Patterson (1994), implicate CD9 as an important regulator of Schwann cell behavior in peripheral nerve.

Additional Information

© 1995 Society for Neuroscience. For the first six months after publication SfN's license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN's website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Mar. 29, 1994; revised June 13, 1994; accepted July 12, 1994. Expert technical assistance was provided by Daryl Jones and Susan Ou. Support was provided by NSF Grant 9006752 and NIH Grant NS02253 to W.D.M., NIH Grant NS30216 to P.H.P., and by an American Heart Association fellowship to M.H.

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