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Published December 15, 2009 | Published
Journal Article Open

Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy


We report optical-resolution photoacoustic microscopy (OR-PAM) for in vivo imaging of amyloid plaques in an Alzheimer's disease mouse model. Validation using conventional fluorescence microscopy and multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window without angiographic contrast agents. OR-PAM, capable of providing both exogenous molecular contrast and endogenous hemoglobin contrast, has the potential to serve as a new technology for in vivo microscopic observations of cerebral plaque deposits.

Additional Information

© 2009 Optical Society of America. Received July 8, 2009; revised November 10, 2009; accepted November 10, 2009; posted November 12, 2009 (Doc. ID 114001); published December 15, 2009. The authors appreciate Prof. James Ballard's close reading of the manuscript. This work was sponsored by National Institutes of Health (NIH) grants R01 NS48283, R01 NS67905, P01 NS32636 (J. M. L.), R01 EB000712, R01 NS46214, R01 EB008085, and U54 CA136398. L. V. W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. For more information, contact J.-M. L. (animal model) or L. V. W. (photoacoustic imaging).

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Published - HuS_2009_Opt_Lett_34_p3899.pdf


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