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Published March 1, 2011 | Published
Journal Article Open

Single-wavelength functional photoacoustic microscopy in biological tissue


Recently, we developed a reflection-mode relaxation photoacoustic microscope, based on saturation intensity, to measure picosecond relaxation times using a nanosecond laser. Here, using the different relaxation times of oxygenated and deoxygenated hemoglobin molecules, both possessing extremely low fluorescence quantum yields, the oxygen saturation was quantified in vivo with single-wavelength photoacoustic microscopy. All previous functional photoacoustic microscopy measurements required imaging with multiple-laser-wavelength measurements to quantify oxygen saturation. Eliminating the need for multiwavelength measurements removes the influence of spectral properties on oxygenation calculations and improves the portability and cost-effectiveness of functional or molecular photoacoustic microscopy.

Additional Information

© 2011 Optical Society of America. Received November 1, 2010; revised December 20, 2010; accepted December 21, 2010; posted February 3, 2011 (Doc. ID 137468); published March 1, 2011. The authors thank Junjie Yao for his help in extracting sO_2 information using the dual-wavelength analysis. This work was sponsored in part by National Institutes of Health (NIH) grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, and 5P60 DK02057933. L. W. has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work.

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