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Published February 15, 1985 | Published
Journal Article Open

DNA sequence of the mouse H-2Dd transplantation antigen gene


The inbred BALB/c mouse has three transplantation antigens, H2-Kd, H2-Ld, and H2-Dd. We present the complete nucleotide sequence of the H2-Dd gene as well as 777 residues of previously unpublished H-2Dd protein sequence. These data complete the sequences of all the BALB/c transplantation antigen genes and permit detailed comparison with each other and with their counterparts from the inbred C57BL/10 mouse. Transplantation antigens may differ from one another by as much as 5%-15% of their amino acid sequence for the external domains. These extensive differences may arise by gene conversion. The H-2D region of the BALB/c mouse encodes the H2-Dd and the H2-Ld genes. Serologic data suggest that at least two additional transplantation antigen molecules, H2-Rd and H2-Md, are encoded in the H-2D region of the major compatibility complex. Paradoxically, gene cloning studies have only identified the H2-Dd and the H2-Ld genes in the H-2D region. A complete DNA sequence of the H2-Dd gene shows that a variety of alternative splice sites exist throughout the gene, which may lead to additional gene products and may explain the multiplicity of H-2D-encoded polypeptides.

Additional Information

© 1985 by the National Academy of Sciences. Communicated by Ray D. Owen, October 3, 1984. We thank Donna Livant for helping to check the H-2Dd gene sequence. We thank Marc Sher and the University of California, Irvine, Proton Decay Group for computer assistance. We thank Tim Hunkapiller for useful discussions. We thank Connie Katz for typing the manuscript. We also thank Mary Spengler, Robert Valas, and Michael Raum for technical assistance. The protein sequence work was supported by Grant AI18556 to R.N. The DNA sequence work was supported by Grant AI9624 (to L.E.H.). B.T.S. was supported by National Institutes of Health Training Grant GM07616. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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