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Published April 2015 | Accepted Version
Journal Article Open

Three-dimensional arbitrary trajectory scanning photoacoustic microscopy


We have enhanced photoacoustic microscopy with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-to-noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO_2) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. We also observed sO_2 dynamics in response to switching from systemic hypoxia to hyperoxia.

Additional Information

© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Manuscript Received: 9 MAY 2014. Manuscript Revised: 27 JUN 2014. Manuscript Accepted: 2 JUL 2014. Version of Record online: 30 JUL 2014. The authors appreciate the close reading of the manuscript by Prof. James Ballard. We also thank Junjie Yao, Liren Zhu, Wenxin Xing, Jinyang Liang, Yong Zhou and Lidai Wang for helpful discussions. This work was sponsored by National Institutes of Health Grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA186567 (NIH Director's Transformative Research Award), R01 CA159959, and R01 EB016963. L.V.W. has financial interests in Microphotoacoustics, Inc. and Endra, Inc., neither of which supported this work. K. Maslov has a financial interest in Microphotoacoustics, Inc.

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