Published May 1975
| Version public
Journal Article
Open
In vitro transformation of lymphoid cells by Abelson murine leukemia virus
Abstract
Cell cultures prepared from fetal murine liver were infected by Abelson murine leukemia virus. After about 2 weeks, proliferating cells of lymphoid morphology appeared in some of the cultures. Addition of 2-mercaptoethanol to the initial culture medium greatly enhanced the appearance of the lymphoid cells. Immunoglobulin determinants were evident on the cells in some cultures. Continuous passage of the cells in certain cultures was possible and the passaged cells could form tumors after animal inoculation. Because Abelson murine leukemia virus is able to induce in vitro malignant transformation of lymphoid cells, it probably causes leukemia by directly affecting cellular growth control.
Additional Information
© 1975 by the National Academy of Sciences. Contributed by David Baltimore, March 7, 1975. We thank Dr. R. Auerbach for helpful discussions, Dr. E. Schneeberger for preparing the electron micrographs and Dr. R. Parkman for performing the cytotoxicity assays. J. Fontaine and C. Bastone provided excellent technical assistance. This work was supported by Grants CA-13472, CA-05616, and CA-14051 from the National Institutes of Health and a contract from the Virus Cancer Program of the National Cancer Institute. C.D.S. was aided by a Cancer Research Scholar Award from the American Cancer Society, Massachusetts Division, Inc. D.B. is an American Cancer Society Research Professor.Files
ROSpnas75.pdf
Files
(1.8 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:98b6d581648ade2bcfc66fe42c8d85e6
|
1.8 MB | Preview Download |
Additional details
Identifiers
- Eprint ID
- 8917
- Resolver ID
- CaltechAUTHORS:ROSpnas75
Dates
- Created
-
2007-10-01Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field