Transducing methyltransferase activity into electrical signals in a carbon nanotube–DNA device
This study creates a device where the DNA is electronically integrated to serve as both the biological target and electrical transducer in a CNT–DNA–CNT device. We detect DNA binding and methylation by the methyltransferase M.SssI at the single molecule level. We demonstrate sequence-specific, reversible binding of M.SssI and protein-catalyzed methylation that alters the protein-binding affinity of the device. This device, which relies on the exquisite electrical sensitivity of DNA, represents a unique route for the specific, single molecule detection of enzymatic activity.
© 2012 The Royal Society of Chemistry. Received 07 Oct 2011, Accepted 19 Oct 2011. First published on the web 20 Oct 2011. We are grateful to the National Institute of Health (GM61077 to JKB), the Office of Naval Research (N00014-09-1-1117 to JKB and CN), the National Science Foundation NIRT (ECCS-07-07748 to CN), and the National Science Foundation (CHE0936923 to AAG) for their financial support.
Published - Wang2012p16694Chem_Sci.pdf