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Published May 1994 | metadata_only
Journal Article

Selective H^+-Dependent Release of Contents from Thymine-Labeled Phospholipid Vesicles by an Adenine-Labeled Polyelectrolyte


Copolymers bearing 5.6, 9, or 11.7 mol % of adenine residues were prepared by radical copolymerization of 2-ethylacrylic acid (EAA) and 9-[[2-(methacryloyloxy)ethoxy]methyl] adenine (MAAd). A double-chain surfactant with thymine in the headgroup was synthesized and used in mixtures with egg yolk phosphatidylcholine (EYPC) to prepare thymine-labeled unilamellar lipid vesicles. Analysis of the pH-dependent behavior of the copolymers revealed that release of contents could be effected from labeled vesicles in competitive experiments in which unlabeled vesicles remained intact. Selectivity was greatest for the 9 mol % copolymer and was reduced at either lower or higher levels of adenine labeling. The selective recognition of the labeled membranes is ascribed to interactions between polymer-bound adenine and surface-bound thymine functional groups.

Additional Information

© 1993 American Chemical Society. Received December 21, 1993; Revised Manuscript Received February 15, 1994. This work was supported by the NSF Materials Research Laboratory at the University of Massachusetts. Financial support by the Direccion General de Investigacion Cientifica y Tecnica for M.B.M. is gratefully acknowledged.

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August 20, 2023
August 20, 2023