Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published February 24, 2017 | public
Journal Article Open

The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport


DNA charge transport chemistry offers a means of long-range, rapid redox signaling. We demonstrate that the [4Fe4S] cluster in human DNA primase can make use of this chemistry to coordinate the first steps of DNA synthesis. Using DNA electrochemistry, we found that a change in oxidation state of the [4Fe4S] cluster acts as a switch for DNA binding. Single-atom mutations that inhibit this charge transfer hinder primase initiation without affecting primase structure or polymerization. Generating a single base mismatch in the growing primer duplex, which attenuates DNA charge transport, inhibits primer truncation. Thus, redox signaling by [4Fe4S] clusters using DNA charge transport regulates primase binding to DNA and illustrates chemistry that may efficiently drive substrate handoff between polymerases during DNA replication.

Additional Information

© 2017 American Association for the Advancement of Science. 19 May 2016; accepted 23 January 2017. Supported by NIH operating grants GM61077 and GM120087 (J.K.B.); NIH operating grants GM65484 and GM118089 (W.J.C.); NIH training grants T32 GM07616 (E.O'B.), T32 GM08230 (M.E.H. and L.E.S.), and T32 CA009582 (A.C.E.); NIH grant S10 RR025677 in support of NMR instrumentation; and the Moore Foundation. Crystallographic data for the p58C Y345F and Y347F mutants have been deposited in the Protein Data Bank under accession numbers PDB 517M and PDB 5DQO, respectively.

Attached Files

Supplemental Material - 23/355.6327.eaag1789.DC1/OBrien.SM.pdf

Accepted Version - nihms848302.pdf


Files (1.8 MB)
Name Size Download all
1.8 MB Preview Download

Additional details

August 19, 2023
August 19, 2023