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Published December 2, 2020 | Supplemental Material + Accepted Version
Journal Article Open

SARS-CoV-2 RapidPlex: A Graphene-Based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring


The COVID-19 pandemic is an ongoing global challenge for public health systems. Ultrasensitive and early identification of infection is critical in preventing widespread COVID-19 infection by presymptomatic and asymptomatic individuals, especially in the community and in-home settings. We demonstrate a multiplexed, portable, wireless electrochemical platform for ultra-rapid detection of COVID-19: the SARS-CoV-2 RapidPlex. It detects viral antigen nucleocapsid protein, IgM and IgG antibodies, as well as the inflammatory biomarker C-reactive protein, based on our mass-producible laser-engraved graphene electrodes. We demonstrate ultrasensitive, highly selective, and rapid electrochemical detection in the physiologically relevant ranges. We successfully evaluated the applicability of our SARS-CoV-2 RapidPlex platform with COVID-19-positive and COVID-19-negative blood and saliva samples. Based on this pilot study, our multiplexed immunosensor platform may allow for high-frequency at-home testing for COVID-19 telemedicine diagnosis and monitoring.

Additional Information

© 2020 Elsevier Inc. Accepted: September 29, 2020; Received in revised form: September 20, 2020; Received: September 1, 2020. This project was supported by the Merkin Institute for Translational Research at Caltech, Emergency COVID-19 Research Seed Funding and COVID-19 Continuation Funding from Tobacco-Related Disease Research Program (TRDRP), TRDRP High Impact Pilot Research Award, National Institutes of Health (5R21NR018271), and the Translational Research Institute through NASA NNX16AO69A. J.T. was supported by the National Science Scholarship from the Agency for Science, Technology and Research (A∗STAR), Singapore. We gratefully acknowledge critical support and infrastructure provided for this work by the Kavli Nanoscience Institute at Caltech. Author Contributions: W.G., R.M.T.-R., and H.L. initiated the concept and designed the experiments; R.M.T.-R., H.L., and J.T. led the experiments and collected the overall data; Y.Y. performed electrode fabrication and characterization; J.M. performed the circuit design and platform test; C.X. contributed to sensor characterization; W.G., R.M.T.-R., and H.L. contributed the data analysis and co-wrote the paper. All authors provided the feedback on the manuscript. The authors declare no competing interests.

Attached Files

Accepted Version - PIIS2590238520305531.pdf

Supplemental Material - 1-s2.0-S2590238520305531-mmc1.pdf


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