Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published September 1, 2005 | Supplemental Material
Journal Article Open

DNA Charge Transport Leading to Disulfide Bond Formation


Here, we show that DNA-mediated charge transport (CT) can lead to the oxidation of thiols to form disulfide bonds in DNA. DNA assemblies were prepared possessing anthraquinone (AQ) as a photooxidant spatially separated on the duplex from two SH groups incorporated into the DNA backbone. Upon AQ irradiation, HPLC analysis reveals DNA ligated through a disulfide. The reaction efficiency is seen to vary in assemblies containing intervening DNA mismatches, confirming that the reaction is DNA-mediated. Interestingly, one intervening mismatch near the thiols promotes an increase in efficiency, which we attribute to increased base dynamics. Hence, here, where the reaction is on the backbone rather than within the base stack, stacking perturbations do not necessarily lead to an inhibitory effect on DNA CT.

Additional Information

© 2005 American Chemical Society. Received June 29, 2005. Publication Date (Web): August 16, 2005. We are grateful to the NIH (GM49216) for financial support. T.T. thanks the Japan Society for the Promotion of Science for a postdoctoral fellowship.

Attached Files

Supplemental Material - ja054306nsi20050803_031451.pdf


Files (294.0 kB)
Name Size Download all
294.0 kB Preview Download

Additional details

August 19, 2023
October 17, 2023